Epidemic Clostridium difficile Strain in Hospital Visitation Dog

5. Hepatic granuloma due to a nymph of Linguatula serrata in a woman from Michigan: a case report and review of the literature. Am J Trop Med Hyg. 1984;33:187–9. 6. A nodular pulmonary lesion due to Linguatula serrata in an HIV-positive man. Two cases of human infestation by larvae of Linguatula serrata. To the Editor: Rates of illness and death from Clostridium difficile–asso-ciated disease (CDAD) and reports of CDAD in persons without traditional risk factors (1) have been increasing. One particular strain of C. difficile has been implicated in outbreaks of CDAD in hospitals in North America and Europe and appears to be spreading internationally at an alarming rate. This strain is classified as ribotype 027, toxinotype III, and possesses genes encoding toxins A, B, and CDT (binary toxin) as well as a deletion in the tcdC gene, which is believed to increase virulence (2). We report this toxin-variant strain of C. difficile in a healthy, 4-year-old toy poodle that visits persons in hospitals and long-term care facilities in Ontario on a weekly basis. C. difficile was isolated from a fecal sample collected in the summer of 2004 as part of a cross-sectional study evaluating pathogen carriage by visitation dogs (3). The isolate was subsequently characterized by ribotyping (4) and by polymerase chain reaction (PCR) detection of genes that encode production of toxins A and B (5). Toxin CDT was confirmed by amplifying the portion of the gene (cdtB) that encodes for the receptor-binding component of the toxin, according to a previously reported protocol (6). As a result, the isolate was classified as ribotype 027, toxinotype III (7), and was found to possess all 3 toxin genes. The tcdC gene deletion was also confirmed with PCR (8). These results indicate that this canine isolate is indistinguishable from the major strain implicated in outbreaks of highly virulent CDAD around the world. According to the infection control practitioner at the hospital the dog visited, CDAD cases were occurring at increased frequency in the facility around the time the dog's fecal specimen was collected. However, patient diagnosis was made solely through fecal toxin testing, and strains were not characterized. The facility has reported only sporadic cases of CDAD in the past few years. This is the first report of this human, epidemic strain of C. difficile in a dog. Many C. difficile strains isolated from animals, including dogs, are indistinguishable from strains associated …